ブラキウリン

ブラキウリン(Brachyurin、EC 3.4.21.32)は、酵素である^[1]^[2]^[3]^[4]^[5]^[6]。この酵素は、幅広い特異性でペプチド結合を加水分解する反応を触媒する。コラーゲンは、N末端から75%の長さで切断される。

この酵素は、シオマネキの仲間であるUca pugilatorの中腸腺から単離された。

出典

^ Hurion, N., Fromentin, H. and Keil, B. (1979). “Specificity of the collagenolytic enzyme from the fungus Entomophthora coronata: comparison with the bacterial collagenase from Achromobacter iophagus”. Arch. Biochem. Biophys. 192: 438-445. PMID 219780.
^ Grant, G.A., Eisen, A.Z. and Bradshaw, R.A. (1981). “Collagenolytic protease from fiddler crab (Uca pugilator)”. Methods Enzymol. 80: 722-734.
^ Welgus, H.G., Grant, G.A., Jeffrey, J.J. and Eisen, A.Z. (1982). “Substrate specificity of the collagenolytic serine protease from Uca pugilator: studies with collagenous substrates”. Biochemistry 21: 5183-5189. PMID 6756469.
^ Welgus, H.G. and Grant, G.A. (1983). “Degradation of collagen substrates by a trypsin-like serine protease form the fiddler crab Uca pugilator”. Biochemistry 22: 2228-2233. PMID 6305411.
^ Klimova, O.A., Borukhov, S.I., Solovyeva, N.I., Balaevskaya, T.O. and Strongin, A.Y. (1990). “The isolation and properties of collagenolytic proteases from crab hepatopancreas”. Biochem. Biophys. Res. Commun. 166: 1411-1420. PMID 2154979.
^ Lu, P.-J., Liu, H.-C. and Tsai, I.-H. (1990). “The midgut trypsins of shrimp (Penaeus monodon). High efficiency toward native protein substrates including collagens”. Biol. Chem. Hoppe-Seyler 371: 851-859. PMID 1963309.